Microneedling Experiment Progress Report — Day 4

Author: Wendy Ouriel, M.S. Cellular Biology — OUMERE Laboratories

Published: OUMERE Research Library

Abstract

This ongoing laboratory investigation replicates OUMERE’s previous microneedling study (previous report here) to conclusively document the effects of repeated dermarolling on human skin. Preliminary observations reaffirm earlier findings of rapid deterioration, structural thinning, and discoloration following even a single microneedling session.

Experimental Design

The study compares three conditions applied to fresh human skin tissue maintained in culture:

Control: Deionized water applied once daily.
Experimental 1: One microneedling session (dermarolling) per day for seven days.
Experimental 2: OUMERE Skin Care regimen daily — No.9 Exfoliant, UVR Concentrate, Advancement, and Serum Bioluminelle.

Samples are incubated at 42°C in nutrient medium for seven days before fixation in Bouin’s solution, dehydration, and embedding in epoxy resin for histological analysis.

Current Observations (Day 4 of 7)

Both the control and OUMERE-treated samples display stable morphology, intact epidermal layers, and smooth, hydrated surfaces. The OUMERE-treated specimen remains firm, with preserved tone and normal coloration.

In stark contrast, the microneedled sample shows significant mechanical disruption even after one session. Surface examination revealed dense crosshatching of punctures resembling pumice texture, grey discoloration, and loss of elasticity.

Microneedled human skin sample day 4 showing pockmarks and greying surface — Microneedled skin shows pockmarked surface, crosshatch pattern, and grey discoloration indicative of tissue oxidation and cellular death.

Each subsequent microneedling session worsened the specimen’s condition despite optimal growth medium. The tissue has become thinner, less resilient, and structurally compromised. The sample’s grey hue and surface collapse indicate cellular oxidation and degradation of collagen and subcutaneous tissue layers.

Preliminary Analysis

Even minimal dermarolling causes substantial physical trauma that extends beyond the epidermis into collagen, muscle, and adipose layers. The mechanical force compromises the extracellular matrix and appears to induce pigment alteration, likely through inflammatory oxidative stress.

Early data suggest that a single microneedling session may biologically age skin tissue by the equivalent of approximately one year, due to collagen fragmentation and cellular necrosis.

Next Steps

At the end of the seven-day treatment phase, the tissues will be fixed, dehydrated, embedded, and sectioned for microscopy. Further updates will document histological comparisons between microneedled, control, and OUMERE-treated specimens.